Lucie Roger

Responsible for the formulation of lipid nanoparticles for encapsulating nucleic acids encoding CRISPR/Cas9 systems

• Designed lipid nanoparticles to encapsulate mRNAs encoding for CRISPR/Cas9-like genome editors
• Produced and characterized CRISPR-like mRNAs
• Optimized lipid nanoparticles formulation on cell lines and patient stem cells

LNP production, mRNA synthesis, purification, QC, flow cytometry, cell culture (K562 & healthy donor stem cells), mRNA electroporation, LNP transfection, PCR, ddPCR

Responsible for the biocompatibility assay of a cell-encapsulated device in a murine model for metachromatic leukodystrophy.

• Performed histological analyses of murine samples, including sectioning, staining, imaging by fluorescence microscopy, and image analysis.

Paraffin embedding, microtome cutting, HE/Masson's trichrome/DAPI/Toluidine staining, BF/fluorescence microscopy, image analysis

Responsible for optimizing a critical parameter in the Upstream process of lentiviral vector production for a CAR-T application.

• Trained in biosafety level 2 & 3 laboratory techniques
• Participated in the production chain of lentiviral vectors for acute lymphoblastic leukemia (UpStream & DownStream)
• Optimized a critical parameter in the production process

Cell culture (HEK293T), lentiviral vector production (flask scale & bioreactor), viral transduction, clarification, ultrafiltration, diafiltration, sterile filtration