Thibaut TABANOU

"Mitochondrial targetting by gold nanoparticles - Therapeutic interest in prostate tumor cell model and evaluation of radiosensitizing properties."

Development and characterization of an in-vitro radiosensitizing mitochondrial-targetting therapeutic strategy of prostatic radioresistant tumor cells (PC3), using functionalized gold nanoparticles coupled with X-Ray radiotherapy.

Preparing for defense (PhD defense scheduled for December, 2024).

• PC3 cell culture
• Clonogenicity
• Cell survival tests (MTT, trypan...)
• Spectrophotometry analysis
• Transmission Electronic Microscopy
• RT-qPCR
• ICPMS (Au quantification)
• Western-Blot and protein quantification
• Immunocytochemistry / Epi-fluorescence microscopy
• Manuscript writing / bibliographic Analyses
• Biochemistry teaching assignments (Clermont-Auvergne University)
• Participation in scientific congresses and events as a
  speaker (oral communications)
• Poster creation (scientific congresses and flash-
  presentations)

"Study of radioprotective properties of hibernating bear serum and its transposition on human cells."

• PC3 and HeLa cell culture
• Clonogenicity analyses
• Cellular survival/cytotoxicity assay (MTT assay)
• Validation of RT-qPCR primers
• Survival test MTT

"Study of the astrocytic expression of the NKP46 receptor in the Experimental Auto-immune Encephalomyelitis
mouse model of multiple sclerosis."

• Astrocytic primary cell culture
• Immunocytochemistry (astrocytic cells)
• Immunohistochemistry (mice spinal cord sections)
• Confocal Microscopy (immunofluorescence)
• Mice Genotyping

"Identification and validation of senescent articular cells specific biomarkers associated with osteoarthritis."

• Chondrocytes and Mesenchymal Stem Cells culture
• In-Vitro Senescence cellular induction
• RT-qPCR
• FACS