Summary
Overview
Work History
Education
Skills
Additional Information
Timeline
Intern

Maha Arafat

Research Engineer
Paris

Summary

Proactive and dynamic researcher with MSc. in biological and chemical Engineering. Acquired hands-on experience in genetic engineering, microbial cultivation, membrane-based separation techniques. Proficient in ( HPLC, GC-MS, SEC, LC-MS, BioLector XT). Eager to learn new methods and skills, and motivated to advance sustainable bioproduction and circular economy.

Overview

3
3
years of professional experience
4
4
Languages

Work History

Research Trianee

VTT - TECHNICAL RESEARCH CENTRE OF FINLAND
02.2025 - 08.2025
  • Genetically engineered E. coli strains for mcl-PHA copolymers production, combining construct design with genome editing (Gibson assembly, recombineering , and electroporation).
  • Applied DBTL iteration to optimize the gene expressions, increasing PHA content by 20% of cell dry weight.
  • Used BioLector XT microbioreactors for real-time monitoring of biomass, pH, and DO.
  • Ran analytical assays (HPLC, GC-MS, and spectrophotometry); documented methods and results for reproducibility.
  • Screened 20 AI-designed enzyme variants and identified top candidates for improved PHA biosynthesis.

Summer Intern

TalTech – Food Tech & Bioengineering Lab
06.2024 - 08.2024
  • Worked with Agrobacterium tumefaciens engineering and mediated transformation.
  • Hands-on Experience with Recombinant DNA/RNA technologies, Golden gate assembly, Logic Gates, and CRISPR/Cas9.
  • Performed Plasmids purification, PCR, DNA extraction , gel electrophoresis.
  • Prepared cultivation media and reagents, competent cells, and worked with flow cytometry.
  • Designed a growth media using a side product of Rhodotorula toruloides respecting circular bioeconomy concepts.

Intern

GENETICS & MOLECULAR BIOLOGY LAB (UNIVERSITY OF DEBRECEN)
02.2022 - 05.2023
  • Investigated the genomic materials of the interspecific hybrids of S. kudriavzevii x S. cerevisiae.
  • Hands-on experience with PFGE- CHEF Mapper, genomic and mitocondrial DNA extraction, and qPCR, PCR-RFLP.
  • Conducted sporulation test, Durham fermentation, and carbohydrates assimilation assay.
  • Followed Good Laboratory Practice (GLP); designed experiments and recorded results aligned with lab protocols.

Education

Master of Science - BIOLOGICAL AND CHEMICAL ENGINEERING FOR A SUSTAINA

AgroParisTech - De L’Université Paris-Saclay
Paris
04.2001 -

Bachelor of Science - Biochemical Engineering

University of Debrecen
Hungary
04.2001 -

Skills

Molecular cloning

Additional Information

Integrated in-situ extraction of microalgal lipids and pigments

  • Designed a full cultivation- extraction (ISPR) flowsheet; compared pervaporation, pulsed electric field, and ultrafilteration; chose biocompatible solvent and suitable membrane.
  • Built a KPI framework defining flux, recovery, selectivity, cell viability, and productivity.
  • Performed trade-off and feasability analysis; mapped mass-transfer limits, water footprint, energy consumptions to identify the optimal operating conditions.

Valorization of food processed wastes

  • Worked on a biorefinery cascade for extracting valuable products from citrus peels, advancing circular bioeconomy goals toward achiving Net Zero.
  • Performed characterization using FTIR, 13 C, 1 H &31P NMR, Size Exclusion chromatography SEC, Liquid Chromatography Mass spectroscopy LCMS, GC-MS, Ultraviolet-visible Spectroscopy UV-Vis, and Differential scanning calorimetry DSC.
  • Completed Life cycle analysis LCA using Open LCA and Eco-invent databases focusing on Carbon and water footprints and global warming potential GWP.
  • Worked with DPPH assay, Klason-lignin assay, Thioacidolysis, and GC-based sugar analysis to analyze and identify the extracted molecules.

Timeline

Research Trianee

VTT - TECHNICAL RESEARCH CENTRE OF FINLAND
02.2025 - 08.2025

Summer Intern

TalTech – Food Tech & Bioengineering Lab
06.2024 - 08.2024

Intern

GENETICS & MOLECULAR BIOLOGY LAB (UNIVERSITY OF DEBRECEN)
02.2022 - 05.2023

Master of Science - BIOLOGICAL AND CHEMICAL ENGINEERING FOR A SUSTAINA

AgroParisTech - De L’Université Paris-Saclay
04.2001 -

Bachelor of Science - Biochemical Engineering

University of Debrecen
04.2001 -
Maha ArafatResearch Engineer